Antibody selection using clonal cocultivation of Escherichia coli and eukaryotic cells in miniecosystems [Applied Biological Sciences]

We describe a method for the rapid selection of functional antibodies. The method depends on the cocultivation of Escherichia coli that produce phage with target eukaryotic cells in very small volumes. The antibodies on phage induce selectable phenotypes in the target cells, and the nature of the antibody is determined…

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