Inverse enzyme isotope effects in human purine nucleoside phosphorylase with heavy asparagine labels [Biophysics and Computational Biology]

Transition path-sampling calculations with several enzymes have indicated that local catalytic site femtosecond motions are linked to transition state barrier crossing. Experimentally, femtosecond motions can be perturbed by labeling the protein with amino acids containing 13C, 15N, and nonexchangeable 2H. A slowed chemical step at the catalytic site with variable…

***

Note from Journals.Today : This content has been auto-generated from a syndicated feed.